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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: FEBS J. 2016 Oct 1;283(21):3898–3918. doi: 10.1111/febs.13895

Figure 7.

Figure 7

Gal/VNPT55 inhibits p65 and Twist1 chromatin binding (a) Schematic of NF-κB binding site in Snail promoter region (a, top panel). Gal/VNPT55 decreases p65 binding activity to Snail promoter region in PC-3 cells (a, bottom graph). (b) RNA collected from PC-3, DU145 and CWR22Rv1 cells were analyzed for BMI-1 expression levels after 24 h treatment (*p<0.05, **p<0.001). (c) Schematic representation of Twist1 binding site in BMI-1 promoter region. (d) DU145 and (e) CWR22Rv1 cells were treated with gal/VNPT55 for 36 h at 10 μM and interaction between Twist1 and BMI-1 promoter measured using chromatin immunoprecipitation (ChIP) assay. BMI-1 promoter fold enrichment was normalized to input controls and presented as mean ± SEM (**p<0.001).