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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Eur J Neurosci. 2016 Oct 16;44(10):2846–2857. doi: 10.1111/ejn.13410

Figure 4.

Figure 4

EYFP colocalizes with MCH neurons but not with orexin (ORX) neurons. Confocal microscopy photomicrographs depict renderings of Z-stacks in XYZ planes. Left panel depicts colocalization of ChR2-EYFP in MCH-immunoreactive neurons, and right panel shows colocalization in orexin-ir neurons. Images were taken from sections from the same rat shown in figure 3 (WT38). Representative neurons are identified with arrowheads or arrows. White arrowheads identify double labeled somata (EYFP+MCH), white arrows identify either single MCH-ir (left panels) or ORX-ir (right panels) neurons, and green arrows identify single-labeled EYFP+ neurons. X axis=green line, Y axis=red line, Z=axis=blue line. Scale bars indicate the distance in microns.