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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Cell Microbiol. 2016 Jul 26;18(12):1815–1830. doi: 10.1111/cmi.12625

Figure 2. Intravitreal injection of AICAR reduced bacterial load and inflammatory mediators in S. aureus-infected eyes.

Figure 2

After 12h of S. aureus infection (5000 cfu/eye), the eyes of C57BL/6 mice (n=6) were treated with AICAR (30 μg/eye, delivered intravitreally in 2μl volume) and mice were sacrificed at 24, 48, and 72h post AICAR treatment. Control eyes received same volume of PBS (vehicle used to dissolve AICAR). Enucleated eyes were crushed in sterile PBS and used for bacterial enumeration (A), and the same lysate was used for quantitation of indicated inflammatory mediators using ELISA (B–D). In another experiment, pooled retinas from two eyes with or without AICAR, treatments (24h time point) were used for Western blot and quantification of p-AMPK (E), and p-ACC (F), as described in Fig. 1 legend. The data shown are mean ± SD of three independent experiments. Statistical analysis was performed using Student’s t-test by comparing S. aureus infected samples with or without AICAR treatment.* p < 0.05, ** p < 0.001, *** p < 0.0001.