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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Cell Microbiol. 2016 Jul 26;18(12):1815–1830. doi: 10.1111/cmi.12625

Figure 3. The anti-inflammatory effects of AICAR in bacterial endophthalmitis are mediated via AMPK activation.

Figure 3

The eyes of C57BL/6 mice (n=6) were pretreated via intravitreal injection of AMPK inhibitor, Compound C (Comp C) or PBS (vehicle) control. After 12h of pretreatment, eyes of Comp C pretreated and two PBS groups were infected with S. aureus (5000 cfu/eye) and 12h post-bacterial challenge, AICAR was injected in Comp C treated and one PBS treated group. All mice were sacrificed after 24h of AICAR treatment, eyes were enucleated and whole eye lysates were subjected to TNF-α, IL-1β and IL-6 ELISA. Each data point represents mean ± SD, and the results are cumulative of two independent experiments. Statistical analysis was performed using one-way ANOVA with Bonferroni’s multiple-comparison test. * p < 0.05, *** p < 0.0001;