Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Nov 22;2:16042. doi: 10.1038/celldisc.2016.42

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

Trans activation requires the N-terminus of EX4. The cAMP signal stimulated by (a) GLP-1R TMD co-expressed with full length or truncated EX4-ECD-M; (b) full-length GLP-1R co-expressed with non-membrane tethered full length or truncated EX4 peptides and (c) full-length GLP-1R co-expressed with membrane-tethered full length or truncated EX4. All signals are indicated as relative to control (EX4-5xGSA-GLP-1R FL WT, labeled C), which was set to 100%. Left, cartoon representations of constructs. ‘−’, absence of EX4 construct. ‘NM’, non-membrane tethered. ‘M’, membrane tethered. Error bars=s.d., all experiments were performed as three independent transfection experiments; two-tailed Student’s t test was used to determine P-values for data points versus GLP-1R construct lacking EX4 (marked by red asterisk): NS>0.05; *⩽0.05; **⩽0.01; ***⩽0.001; ****⩽0.0001.