Table 1.
Salmonella and non-Salmonella strains used to evaluate the specificity and sensitivity of the developed PCR system.
| Strain | Serovar/Species | Source | flhB-PCR result (182 bp/379 bp) | |
|---|---|---|---|---|
| Salmonella | C50041 | Enteritidis | Laboratory stock | –/+ |
| C50336 | Enteritidis | Laboratory stock | –/+ | |
| S06004 | Pullorum | Laboratory stock | +/– | |
| 6508 | Pullorum | Isolate from chicken | +/– | |
| SG9 | Gallinarum | Wigley et al., 2005 | +/– | |
| SL5928 | Dublin | Laboratory stock | –/+ | |
| T3 | Uganda | Cai et al., 2016 | –/+ | |
| T9 | Meleagridis | Li et al., 2016 | –/+ | |
| T8 | Anatis | Li et al., 2016 | –/+ | |
| G2 | London | Cai et al., 2016 | –/+ | |
| ZX | Rissen | Cai et al., 2016 | –/+ | |
| Y7 | Derby | Cai et al., 2016 | –/+ | |
| Y8 | Typhimurium | Li et al., 2016 | –/+ | |
| C500 | Choleraesuis | Laboratory stock | –/+ | |
| ZH65 | Indiana | Cai et al., 2016 | –/+ | |
| ZH5 | Sinstorf | Laboratory stock | –/+ | |
| ZH10 | Newlands | Isolate from cattle | –/+ | |
| ZH24 | Muenster | Laboratory stock | –/+ | |
| ZH82 | Yoruba | Isolate from pig | –/+ | |
| G449 | Dumfries | Laboratory stock | –/+ | |
| G241 | Kentucky | Laboratory stock | –/+ | |
| G382 | Agona | Laboratory stock | –/+ | |
| ZH35 | Thompson | Cai et al., 2016 | –/+ | |
| P192 | Senftenberg | Laboratory stock | –/+ | |
| G439 | Blockley | Laboratory stock | –/+ | |
| G86 | Inchpark | Laboratory stock | –/+ | |
| P122 | Virchow | Laboratory stock | –/+ | |
| P74 | Farsta | Laboratory stock | –/+ | |
| G85 | Dabou | Laboratory stock | –/+ | |
| Non-Salmonella | H37Rv | Mycobacterium tuberculosis | ATCC 27294 | –/– |
| 11168 | Campylobacter jejuni | ATCC 700819 | –/– | |
| 110 | Campylobacter jejuni | Isolate from chicken | –/– | |
| S19 | Brucella abortus | Laboratory stock | –/– | |
| EGDe | Listeria monocytogenes | ATCC BAA-679 | –/– | |
| JS15 | Listeria monocytogenes | Isolate from sheep | –/– | |
| 1314 | Escherichia coli | Isolate from chicken | –/– | |
| 1352 | Escherichia coli | Isolate from chicken | –/– |