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Confocal laser scanning microscopy images of three replicate S. aureus biofilms grown for 48 h in TSB media and transferred to either fresh media (A–C) or 0.1% Tween 80 (D–F) followed by growth for 24 h. SYTO 60 (red) stains living bacteria while TOTO-1 (green) stains dead bacteria and extracellular DNA. Scale bar = 20 μm.
