Figure 6.

The effect of pre-incubation with insulin on U46619 constriction is dependent on PI3K. Chorionic vein rings were pre-incubated (30 min, 5%CO₂, 37°C) in absence (control) or presence of 10 nM insulin and/or 1 mM tetraethylammonium (TEA; A), 100 nM iberiotoxin (IbTx; B) or 30 nM wortmannin (C). After these treatments, vessels were exposed to U46619. In (D), the effects are shown of treatments on maximal constriction induced by 1 μM (1 × 10⁻⁶ M) U46619. ^*P < 0.05 vs. values in the absence of insulin. ^†P < 0.05 vs. values in insulin-treated vessels. Values are mean ± S.E.M. (n = 5–7).