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Venn diagram of growth impaired genes observed for growth on TY and VMM-mannitol. Growth impaired genes observed uniquely on TY or VMM were assigned to TY-growth impaired or VMM-growth impaired. Growth impaired genes observed in both treatments were assigned to the CFG. Genes without potential mariner insertion sites, or which had highly similar sequence redundancy in the RLV3841 genome were discounted.
