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. 2016 Nov 22;7:1873. doi: 10.3389/fmicb.2016.01873

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Copyright © 2016 Perry, Akter and Yost.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of plasmid growth impaired genes by Riley functional classification across RLV3841 megaplasmids. Plasmid borne genes observed to be growth defective or essential for growth on both TY and VMM-Mannitol were grouped. The contribution of growth defective or essential genes to megaplasmid stability, or growth of RLV3841 at an organism level, cannot be discriminated by INSeq analysis alone; therefore, the grouped growth defective and essential genes were assigned to plasmid growth impaired. Riley functional classifications were assigned to PGI genes for comparison of PGI profiles between megaplasmids.