Figure 7.
Selective CD28 blockade with FR104 control in vitro Ag primed Tfh responses more effectively than belatacept. (A) Flow cytometry plot of representative of human tonsil cell suspension showing gates used to sort pre-Tfh (CD4+CXCR5+ICOS+) and Tfh (CD4+CXCR5hiICOShi). (B) Proliferative responses to autologous B cell plus Staphylococcal enterotoxin B after 8 days presented as percentages of pre-Tfh and Tfh compared with control conditions under either FR104 (blue circles) or belatacept (red circles). Each pair of connected points represents a paired experiment (n=10). The Wilcoxon matched-paired signed rank test was used. *P<0.05.