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. 2016 Apr 14;27(12):3611–3627. doi: 10.1681/ASN.2015080877

Figure 2.

Figure 2.

RAAS inhibition increases the number of labeled cells of renin lineage in the juxtaglomerular compartment in non-diseased mice renin/red fluorescent protein co-staining. Confocal microscopy showing co-staining for renin (green color, first column) and the RFP reporter (red color, middle column) as single panels. A merge of the two panels creates a yellow color if renin and RFP staining co-localize (right column). Nuclei stain blue with DAPI. The quantitative data is shown in panel D. (A) Non-diseased mice given water: When healthy, non diseased Ren1cCreERxRs-tdTomato-R mice were given tamoxifen, and given water alone to drink for 25 days, renin (A, i) and RFP staining (A, ii) were restricted to co-localized in the juxta-glomerular compartment (JGC) (A, iii). (A, iv – A,vi) Higher magnification images of the insets shown in the figures above. (B) Non-diseased mice given enalapril: Administering the ACE inhibitor enalapril in the drinking water for 25 days to healthy, non-diseased Ren1cCreERxRs-tdTomato-R CoRL reporter mice increased the number of cells in the JGC staining for renin (B, i) and RFP (B, ii). Renin and RFP staining merged, and was restricted to and co-localized in the JGC (B, iii). Higher magnification images of the JGC insets are shown for renin staining (B, iv), RFP staining (B, v) and merged (B, vi). (C) Non-diseased mice given losartan: non-diseased Ren1cCreERxRs-tdTomato-R CoRL reporter mice treated with ARB losartan in drinking water for 25 days increased the number of cells in the JGC staining for renin (C, i) and RFP (C, ii). The majority of cells merged. Higher magnification images of the JGC are shown for renin staining (C, iv), RFP staining (C, v) and merged (C, vi). (D) Quantitative Data: Quantification of the percentage of all cells in the kidney cortex expressing RFP showed a significant increase in healthy, non-diseased animals given either enalapril or losartan compared to water alone control mice. Original magnification is ×400. Data presented as the mean±SD. These results show that in non-diseased reporter mice, RAAS inhibition increases the number of renin stained cells, and labeled cells of renin lineage and significantly increased the percentage of cells in the total kidney cortex cell population that comprised RFP+CoRL compared to mice given water alone. Data are represented as mean ±SD.