Figure 4.

RAAS inhibition increases the number of labeled CoRL in the JGC and migration of fate-mapped CoRL in FSGS mice. (A–C) Confocal microscopy of co-staining of BrdU (green, left column), red fluorescent protein (RFP, red, middle column) and DAPI (blue), and merged images (yellow, right column) (A) Enalapril in non-diseased mice: (A, i) BrdU stained cell detected in the JGC; (A, ii) RFP stained cells in the JGC; (A, iii) BrdU stained cell co-localizes with RFP⁺ cell in the JGC (arrow head). (A, iv–vi) high power magnification of insets shown in figures above (B) Losartan in non-diseased mice: (B, i) BrdU stained cells detected in the JGC; (B,ii) RFP stained cells representing labeled cells of renin lineage; (B, iii) several RFP⁺ cells co-stain with BrdU (arrow heads). (B, iv–vi) high power magnification of insets shown in figures above (C) Enalapril in FSGS mice: (C, i) BrdU staining was detected in cells in the JGC (inset) and also in other cells in the tubule-interstitium; (C, ii) the number of RFP stained cells are higher in the JGC; (C, iii) BrdU co-stains in a subset RFP⁺CoRL in the JGC (arrow heads), but not RFP⁺CoRL in the IGC. (C, iv–vi) High power magnification of insets shown in figures above. (D) Losartan in FSGS mice: (D, i) BrdU staining was detected in cells in the JGC (inset); (D, ii) an increase in the number of RFP stained cells in the JGC (inset); (D, iii) BrdU co-staining in RFP⁺CoRL in the JGC (arrow head), but not RFP⁺CoRL in the IGC (Arrows). (D, iv–vi) high power magnification of insets shown in figures above.