Figure 4.

IL-17F–producing CD4⁺ T cells aggravate renal tissue injury in Rag1^−/− mice. (A) Representative photographs of PAS-stained kidney sections at day 8 of NTN from RAG1^−/− control mice, nephritic RAG1^−/− mice repopulated with 1.5×10⁶ wild-type CD4⁺ T cells, and nephritic RAG1^−/− mice repopulated with 1.5×10⁶ IL-17F^−/− CD4⁺ T cells (original magnification ×400). (B) Quantification of glomerular crescent formation, interstitial area, and glomerular sclerosis at day 8 of NTN of RAG1^−/− controls (n=2), nephritic RAG1^−/− mice repopulated with wild-type CD4⁺ T cells (n=7), and nephritic RAG1^−/− mice repopulated with IL-17F^−/− CD4⁺ T cells (n=8). (C) BUN levels, serum creatinine, and ACR of the aforementioned groups 8 days after induction of nephritis. (D) Quantification of tubulointerstitial GR-1⁺ cells, tubulointerstitial F4/80⁺ cells, and glomerular MAC-2⁺ cells of the aforementioned groups 8 days after induction of nephritis. Symbols represent individual data points with the mean as horizontal line. *P<0.05.