Figure 3.

lncRNA-LET expression inhibited EMT and canonical Wnt/β-catenin pathway in part. (a) Western blot was performed to assay the molecular marker levels of EMT. (b) Western blot was performed to assay the protein level of nuclear β-catenin in LAC cell lines. The relative protein levels were normalized to β-actin. A549 cells were transfected with lncRNA-LET-shRNA or NC-shRNA and H1299 cells were transfected with lncRNA-LET-shRNA or NC-shRNA. Relative protein expression was quantified by the Image J software. (c) RT-PCR was performed to assay the mRNA level of nuclear β-catenin in paracarcinoma tissue (P) and tumor tissue (T). Data are presented as the mean ± SD of three independent experiments. ^∗∗ P < 0.01; ^∗ P < 0.05.