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. 2016 Nov 17;7:13479. doi: 10.1038/ncomms13479

Figure 9. FGF21 gene invalidation reduces the effects of GW9508 treatment in mice.

Figure 9

Wild-type and FGF21-null mice were fed a control diet (white and grey bars, respectively) or supplemented with GW9508 (blue and black bars, respectively) for 7 days (n=5). (a) Relative mRNA levels of thermogenesis-related and adipogenic genes in iBAT (c) and iWAT. Representative optical microscopy images of H&E staining (scale bar, 125 μm), the relative lipid content and UCP1 protein levels in iBAT (b) and iWAT (d), and the percentage of multilocular adipocytes in iWAT. Bars are means+s.e.m. (*P<0.05, **P<0.01 and ***P<0.001 relative to untreated control mice of each genotype; +P<0.05, ++P<0.01 and +++P<0.001 relative to same treatment of the wild-type group; analysis of variance with Tukey's post hoc test).