Figure 1.

Setup for culturing organotypic slices directly on HD-MEAs. (A) A slice cultivation chamber was designed to attach to an HD-MEA chip, hold culture media, and allow for gas exchange. The cultivation chamber was affixed to a custom rotation rack using the two fixing holes. (B) The cultivation chamber was equipped with a red rubber O-ring, which was used to form a seal with the plastic ring around the HD-MEA. (C) A yellow cap with a sterile filter inside allowed for gas exchange and preserved sterile conditions inside the chamber. (D) A cultivation chamber with the HD-MEA attached was affixed onto the rotation rack by using the two holes (one on the side, and the other on the back). (E,F) The rotation rack can hold multiple slice cultivation chambers at the same time and provides a continuous low-speed rotation inside an incubator.