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. 2016 Nov 22;7:1728. doi: 10.3389/fpls.2016.01728

FIGURE 5.

FIGURE 5

AN3 and EMB71/YDA are Involved in Sugar Metabolism and/or Sugar Signaling. (A) Representative 5% glucose treatment accelerates AN3 protein degradation. The 12-day-old 35S:AN3:3X:GFP seedlings grown on solid MS medium with 1% sucrose and 5% glucose. Materials were from at least 10 independently propagated lines. (B) Representative 5% glucose treatment stabilizes EMB71/YDA protein. The 12-day-old PHB:EMB71/YDA:GFP seedlings grown on solid MS medium with 1% sucrose and 5% glucose. Materials were from at least 10 independently propagated lines. (C) Representative 5% glucose can restore the an3-4 delayed flowering. Materials were grown on MS medium with 5% glucose for 4 weeks under long light (16 h light/8 h dark). Seedlings were from the same plate. Magnifications are the same. (D) Bar graph exhibiting the difference in the rosette leaf number between Wild-type (Col-0) and an3-4 seedlings grown on solid MS medium with 1% sucrose and 5% glucose for 4 weeks under long light (16 h light/8 h dark). Error bars represent SD (n = 12). Heteroscedastic t-test analysis showed significant differences (∗∗∗P < 0.001). Experiments were repeated two times with similar results (biological replicates). (E) Longitudinal sections of roots of 8-day-old Ler (a) and yda-1 (b) seedlings. Seedlings were from the same plate. Magnifications are the same. Results are typical of those for many seedling. The arrow indicates starch grains.