Table 3.

Properties of BphP-based NIR FPs.

NIR FP	Exa	Emb	ε at peakc	Φd	BphP	Ref.e
IFP1.4	684	708	92	0.077	DrBphP	[86]
Wi-Phy	701	719	93	0.047	DrBphP	[87]
IFP1.4rev	685	708	131	0.087	DrBphP	[88]
IFP2.0	690	711	98	0.081	DrBphP	[89]
iRFP713	690	713	98	0.063	RpBphP2	[63]
iRFP720	702	720	96	0.06	RpBphP2	[90]
iRFP713/V256Cf	662	680	94	0.145	RpBphP2	[91]
iRFP682	663	682	90	0.113	RpBphP6	[90]
iRFP702	673	702	93	0.082	RpBphP6	[90]
iRFP670f	643	670	114	0.111	RpBphP6	[90]
mIFP	683	704	82	0.084	BrBphP	[84]
iBlueberryf	644	667	80	0.08	BrBphP	[92]
GAF-FPg	635	670	50	0.073	RpBphP1	[93]
BphP1-FP/C20Sf	639	670	82	0.139	RpBphP1	[94]
AphB variants	~695	~720	~80	~0.06	AphB	[95]

^aExcitation maximum in nm

^bEmission maximum in nm

^cExtinction coefficient at peak in units of mM^-1cm^-1 calculated based on BV absorbance

^dFluorescence quantum yield

^eSource of data

^fBV covalently binds to Cys256 in the GAF domain (the numbering is based on iRFP713)

^gGAF domain only