Figure 1.

(a) Setup of IVM of cremaster post-capillary venules in a live mouse. The cremaster muscles are surgically exposed and perfused with a physiological buffer under an objective, and images are recorded using laser scanning confocal microscope or spinning-disc confocal microscope. (b) 3D image of IVM shows the adhesion of neutrophil membrane-formed nanovesicles to endothelium 3 h after introscrotal injection of TNF-α. The nanovesicles were labeled by DiO dyes (green) and the vessel was stained with Alexa-Fluor-647-anti-CD31 (pink). The image B is reproduced and permitted from the reference ⁷.