Figure 7.

A. In vivo microendoscopy of murine ovarian micrometastatic carcinoma showing selective binding and fluorescence activation of the activatable Cetuximab-BPD PIC upon cellular internalization and proteolytic digestion. Activation is highest at 8-24 hrs following intraperitoneal administration in disease-bearing mice, whereas activation of the PIC is not observed in the “no tumor” control mice (scale bars are 100 μm). B. Peritoneal distribution of BPD fluorescence in mice with disseminated ovarian tumors administered with free BPD (top) or the activatable Cetuximab-BPD PIC. The PIC (bottom) demonstrates selectivity and little activation at disease-free organs, such as the colon (white dotted line), as compared to the non-specific binding of free BPD (top). Figure adapted from Spring et al ¹⁴⁶.