Table 4.
Chlorophyll content, stomatal conductance (gs), N and C content, and N and C isotope composition (δ15N and δ13C, respectively) in four durum wheat genotypes grown under ambient or elevated [CO2] and well-watered or severe water stress conditions (100 vs. 30% pot capacity).
| Genotype | Ambient [CO2] | Elevated [CO2] | PC | PW | PC × W | |||
|---|---|---|---|---|---|---|---|---|
| Well-watered | Water stressed | Well-watered | Water stressed | |||||
| Chlorophyll (SPAD units) | Mexa | 55.8 | 55.0 | 52.7 | 52.7 | 0.100 | 0.790 | 0.807 |
| Regallo | 50.6 | 43.1 | 46.6 | 45.8 | 0.877 | 0.351 | 0.439 | |
| Burgos | 56.6 | 53.2 | 53.3 | 58.4 | 0.661 | 0.683 | 0.076 | |
| Ramirez | 52.7 | 51.6 | 52.8 | 49.0 | 0.532 | 0.230 | 0.500 | |
| gs(mmol m−2 s−1) | Mexa | 81.9 | 55.0 | 223.5 | 91.5 | 0.112 | 0.150 | 0.323 |
| Regallo | 101.8 | 82.3 | 64.4 | 32.2 | 0.265 | 0.499 | 0.866 | |
| Burgos | 245.0 | 96.7 | 247.0 | 30.7 | 0.390 | <0.001 | 0.362 | |
| Ramirez | 44.0 | 52.5 | 151.3 | 57.2 | 0.160 | 0.270 | 0.194 | |
| N (%) | Mexa | 4.39 | 4.67 | 4.10 | 4.54 | 0.491 | 0.257 | 0.785 |
| Regallo | 4.68 | 4.33 | 4.37 | 3.78 | 0.017 | 0.011 | 0.424 | |
| Burgos | 4.89 | 4.66 | 4.15 | 3.97 | 0.013 | 0.398 | 0.918 | |
| Ramirez | 3.81 | 4.22 | 4.16 | 4.43 | 0.521 | 0.437 | 0.872 | |
| δ15N(‰) | Mexa | 2.71 | 2.74 | 3.52 | 3.59 | 0.060 | 0.895 | 0.950 |
| Regallo | 3.78 | 3.27 | 3.55 | 3.58 | 0.947 | 0.685 | 0.646 | |
| Burgos | 2.45 | 2.67 | 3.69 | 3.47 | 0.018 | 0.997 | 0.547 | |
| Ramirez | 2.69 | 2.99 | 3.23 | 2.73 | 0.508 | 0.641 | 0.084 | |
| C (%) | Mexa | 41.8 | 41.8 | 41.3 | 41.9 | 0.490 | 0.368 | 0.439 |
| Regallo | 39.9 | 38.0 | 40.4 | 41.1 | 0.064 | 0.476 | 0.165 | |
| Burgos | 41.9 | 41.4 | 41.8 | 41.3 | 0.877 | 0.590 | 0.968 | |
| Ramirez | 41.4 | 40.7 | 40.9 | 42.1 | 0.416 | 0.658 | 0.115 | |
| δ13C(‰) | Mexa | −32.3 | −31.4 | −56.9 | −56.4 | <0.001 | 0.546 | 0.851 |
| Regallo | −33.5 | −32.2 | −54.4 | −54.0 | 0.001 | 0.850 | 0.927 | |
| Burgos | −32.8 | −32.4 | −46.1 | −46.3 | 0.004 | 0.979 | 0.940 | |
| Ramirez | −31.9 | −32.6 | −59.1 | −56.4 | <0.001 | 0.752 | 0.594 | |
Significant effects for elevated [CO2] (C), water stress (W) and their interaction (C × W) were determined by two-factor ANOVA (P). Significant P values are marked in bold (P < 0.05).