TABLE 4.
Single-cell RT-PCR results from 37 neurons from mice P14–21
| Grin1a | Grin2aa | Grin2ba | Grin2ca | Grin2da | |
|---|---|---|---|---|---|
| Interneuron marker | |||||
| PV expressing | 100% | 78% | 78% | 11% | 67% |
| SOM expressing | 95% | 81% | 86% | 0% | 81% |
| NPY expressing | 86% | 75% | 86% | 4% | 79% |
| CCK expressing | 100% | 57% | 86% | 0% | 100% |
| Firing properties | |||||
| Fast spiking | 100% | 50% | 75% | 25% | 50% |
| Regular spiking | 88% | 73% | 85% | 0% | 82% |
| Soma location | |||||
| slm | 80% | 80% | 60% | 0% | 60% |
| so | 94% | 81% | 88% | 6% | 81% |
| sr | 87% | 60% | 93% | 0% | 87% |
| All interneurons | |||||
| Detection count | 33 | 26 | 31 | 1 | 29 |
| Percentage | 89% | 70% | 84% | 3% | 78% |
Percentage of cells.
After harvesting the intracellular contents of each recorded cell, soma localization was evaluated using fluorescence microscopy and assigned to stratum oriens (so), stratum pyramidale (sp), stratum radiatum (sr), stratum lacunosum moleculare (slm), stratum granulosum (sg), or hilus. Cells were categorized as fast spiking if they sustained high-frequency (>150 Hz) action potential discharge with little or no decrement in spike amplitude during a strong depolarizing current pulse of long duration (1 nA, 800–1000 ms) or regular spiking if they exhibited significant accommodation in spike frequency and decreasing spike amplitude during the same depolarizing current injection. See Supplemental Table S2 for explicit results from all cells.