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. 2016 Dec;359(3):411–419. doi: 10.1124/jpet.116.232835

Fig. 6.

Fig. 6.

Comparison between BK and naloxone for induction of functional competence. (A and B) Cultures of peripheral sensory neurons were pretreated with BK (10 μM, 15 minutes), naloxone (1 μM, 3 hours), or a combination of naloxone (3 hours) + BK (15 minutes). Functional competence of MOR (A) and DOR (B) was by measuring DAMGO (1 μM) (A)- or DPDPE (100 nM) (B)-mediated inhibition of PGE2 (1 μM)-stimulated cAMP accumulation as described in Methods. (C and D) Cultures of peripheral sensory neurons were pretreated with BK (10 μM, 15 minutes) or naloxone (1 μM, 3 hours). After pretreatment, concentration response curves to DAMGO (C) or DPDPE (D) were obtained measuring inhibition of PGE2-stimulated cAMP accumulation. Data represent the mean ± S.E.M. of 3 or 4 experiments and are presented as the percentage of the control PGE2-stimulated cAMP levels (for some data points, error bars are contained within the size of the symbol). *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control PGE2-stimulated cAMP levels.