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. 2016 Dec;359(3):401–410. doi: 10.1124/jpet.116.236158

Fig. 3.

Fig. 3.

Effects of cimetidine on B-to-A flux of atenolol and metformin in hOCT2/hMATE1-expressing MDCK cells. Cells were incubated in KRH buffer containing atenolol (1 μM) or metformin (5.5 μM) in the basal chamber. The pH in the apical and basal chambers was maintained at 6.0 and 7.4, respectively. B-to-A flux of atenolol and metformin was measured in the absence or presence of 2 μM or 10 μM of cimetidine. Cimetidine was added to the basal (A, D), apical (B, E), or both chambers (C, F). At various time points, 50 μl of sample was taken from the apical chamber and replenished with an equal volume of fresh KRH buffer. Experiments were performed in two to three individual Transwell apparatuses and repeated twice. Data were presented as mean ±S.D. (n = 6) of all apparatuses with acceptable TEER values. The B-to-A flux of atenolol and metformin in MDCK-hOCT2/hMATE1 cells in the presence of cimetidine was compared with that in the absence of cimetidine. ***P < 0.001, **P < 0.01, *P < 0.05 indicates significant difference from the no inhibitor control.