Figure 2.

April promotes survival of acute myeloid leukemia cells in NR but not in CR patients. Acute myeloid leukemia cells were treated for 24h with 10µM of cytosine arabinoside (AraC) in the presence or absence of rhAPRIL and rhBAFF. After 24h, the viability of cells was determined by flow cytometry (FACSCalibur, BD) using Propidium Iodide (PI, Becton Dickinson). Graphs are presenting the summary of analyses of: (A) changes in viability of AML cells after incubation in presence or absence of AraC; (B) changes in viability of AML cells after AraC incubation in presence or absence of APRIL in CR (complete remission, left) and NR (non-responders, right) patients; (C) frequencies of viable AML cells after AraC incubation in presence of BAFF in CR (left) and NR (right) patients.