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. 2016 Nov 22;6:37458. doi: 10.1038/srep37458

Figure 5. Rapid intracellular bioprotection of betaine.

Figure 5

(A) Post-thaw efficiency of Hela cells after incubation for different periods in medium containing 0.5% (blue), 1% (red), 2%, (green) of betaine. Post-thaw survival efficiency of (B) GLC-82 cells, (C) Hela cells and (D) MCF-10 cells after cryopreservation using betaine (red), trehalose (blue), and trehalose + 10% DMSO (purple) with ultrarapid freezing protocol. Value = mean ± standard deviation, n ≥ 3.