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. 2016 Nov 22;6:37502. doi: 10.1038/srep37502

Figure 1. Establishment and validation of a Nanoluciferase (NLuc)-based high-throughput screening method of NPP inhibition.

Figure 1

(A) Representative kinetic data from screening method. RBCs infected (iRBC) with Nluc expressing parasites were treated with NPPB prior to adding sorbitol to inhibit iRBC lysis and release of NLuc. DMSO vehicle (100% lysis) and PBS (sorbitol replacement, no lysis) controls are also shown. Dashed lines denote 15 and 28 minutes post sorbitol addition. (B and C) Relative light units (RLU) as a percentage relative to DMSO average at 15 (B) and 28 (C) minutes after addition of sorbitol for each drug treatment. Z′ values for each treatment are listed above the x-axis. (D) Graph of %RLU of NPPB and control-treated iRBC at 15 minutes versus 28 minutes post sorbitol addition.