Table 2. The fold change in the EC50 of parasite growth following drug treatment between 3D7 parasites and two independently transfected lines expressing yeast DHODH (yDHODH).
| EC50 for Parasite Growth |
Fold Increase |
||||
|---|---|---|---|---|---|
| 3D7 | yDHODH #1 | yDHODH #2 | yDHODH #1 | yDHODH #2 | |
| ATV | 176 pM (130–230) | >1.25 μM | >1.25 μM | >7100 | >7100 |
| DSM-1 | 64.8 nM (55–77) | >6.25 μM | >6.25 μM | >96 | >96 |
| NPPB | 54.2 μM (43–68) | 58.6 μM (48–72) | 60.5 μM (53–69) | 1.1 (p =0.6360) | 1.1 (p = 0.4643) |
| Furosemide | 116 μM (97–140) | 127 μM (100–160) | 107 μM (89–130) | 1.1 (p =0.5706) | 0.9 (p = 0.5556) |
| MMV020439 | 278 nM (230–340) | 1.79 μM (1.5–2.2) | 1.66 μM (1.3–2.1) | 6.4 (p =0.0002) | 6.0 (p = 0.0003) |
| MMV007571 | 1.04 μM (0.92–1.2) | 35.4 μM* (24–63) | 40.2 μM * (26–87) | 34 (p =0.0031) | 39 (p = 0.0054) |
The EC50 values (in bold) were calculated from the pooled data from three independent replicates of three technical replicates each. 95% confidence intervals are listed in parenthesis. *Denotes values extrapolated from regression where inhibition did not reach 50%. P-values for fold change (comparing EC50 from 3D7 with corresponding yDHODH) were calculated using a two-tailed Student’s t-test with Welch’s correction, p-values in bold signify <0.05.