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. 2016 Nov 22;6:37454. doi: 10.1038/srep37454

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Fluorescence microscopy of Hrr. lacusprofundi biofilm development. Images of cells stained with acridine orange (cellular plus extracellular DNA) or 4′,6-diamidino-2-phenylindole (extracellular DNA) compared to differential interference contrast microscopy images. Extracellular DNA in biofilms commenced around day 7 and continued to grow through day 14. The scale bar represents 10 μm. Abbreviations: DICM, differential interference contrast microscopy; FM, fluorescence microscopy; AO, acridine orange; DAPI, 4′,6-diamidino-2-phenylindole.