Figure 3. BCH treatment decreased gluconeogenesis but increased ketogenesis in C57BL/6J mice.

C57BL/6J mice were challenged with BCH (0.7 g/kg) for 6 h. Soluble fractions were then taken from liver homogenates by differential centrifugation. (a) GDH activity, (b) level of glutamate, (c) NAD+/NADH ratio, and (d) level of ketone bodies were measured with corresponding assay kits. (e) Mice were treated with 0.7 g/kg BCH for 6 h and then, challenged with pyruvate (2 g/kg). Levels of glucose in blood were measured at 0, 15, 30, and 90 min after pyruvate injection. Data were represented as means ± SEM from two independent experiments (n = 5 per group). *p < 0.05, **p < 0.01 vs. BCH-untreated group.