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. 2016 Nov 22;5:37468. doi: 10.1038/srep37468

Figure 5. BCH protected HF/HFr-induced hepatic insulin resistance in C57BL/6J mice.

Figure 5

(a) Liver proteins were extracted with RIPA buffer. Levels of signaling molecules related to ER stress and inflammation were analyzed by immunoblotting with the appropriate antibodies. (b) Relative expression of genes related to inflammation was determined through real time-PCR. (c) Macrophages in liver tissues were stained with anti-F4/80 antibodies. Arrows designated stained macrophages. (d) Levels of P-Akt and P-IR were analyzed by immunoblotting with anti-P-Akt and anti-P-IR antibodies. (e) SIRT1 level were measured by immunoblotting with anti-SIRT1 antibody. SIRT 1 activity was determined by SIRT1 activity assay kit. Full-length blots are presented in Supplementary Fig. 8. Data were represented as means ± SEM of eight mice per group. *p < 0.05, **p < 0.01, ##p < 0.01 vs. BCH-untreated HF/HFr group.