Figure 6. BCH protected HF/HFr-induced hepatic injury in C57BL/6J mice.

(a) Levels of alanine transaminase (ALT) and aspartate transaminase (AST) in plasma were measured by using an autochemical analyzer. (b) Relative expressions of genes related to liver fibrosis were investigated through real time-PCR (c) Paraffin sections of liver tissue were stained with Sirius Red Solution. Arrows designated stained collagen. (d) Fibrosis stage and (e) NAFLD activity score (NAS) (steatosis/inflammation/ballooning degeneration) were determined using the clinical criteria. Data were represented as means ± SEM of eight mice per group. *p < 0.05, **p < 0.01 vs. BCH-untreated HF/Hfr group.