Table 1. Sequences of primers and PCR condition.
Genes | Exon | Forward | Reverse | Hybridation temp. (°C) |
---|---|---|---|---|
CNGB3 | 5 | GTGAGAACATGCGGTGTTTG | CAAAGATGGGCAAATGATCC | 58 |
CNGB1 | 23 | AGAGACTCCGCCTCTCACTC | GGGGCAGACACGAAGATG | 58 |
ABCA4 | 15 | AGCACATGGAGTGTGCGTAG | TGCCCCTGTACATTTTAGCC | 58 |
NR2E3 | 6 | TCTGAGCCTCTGGCTGATGTCA | AGAAGGGAGTCCAGCCTCAC | 60 |
RPE65 | 4 | GACTTGATGAGGACACATAG | CAGTCCAGTAATTTTCAAGC | 60 |
RPE65 | 6 | TTCAAGGGGTAGTGATGACC | GCACAAAATGCTATTCTGAC | 60 |
RPE65 | 11 | CTTAGGAGCCAAGACTTAAG | GAGGAAACTCAAATGCTACG | 60 |
PROM1 | 12 | ctccagccttagtccagcag | gtcccatcacagcaggatct | 58 |
EYS | 29 | AATCTGCTTCTGGCTTTGTTT | GCCCCACTAGCCAGAAAATA | 58 |
PDE6B | 7 | CCTGCACACAGACATCCAGT | TGGCAGAGACAAGGAGAAGC | 58 |
FAM161A | 3 | TGGTCACATACAACTGAAAGTATAACA | GCTTCTGTTCCTCCCTTGCT | 60 |
CERKL | 8 | GGGAACTGAAGAGAAAGTGAGG | GGCTGAGTGAGTAGTTGTTTGC | 58 |
LRP2 | 69 | TTTTTGCTCCCCATCTTCTG | AATTCAGCAGGTGGGAGTTG | 58 |
PPIG | 14 | CATTTCCCCAATTCTTTTTCTT | GATTGTGCGCGTCTATCCTT | 58 |
CWC22 | 10 | GCTCCTGGAAAGACCAACAG | GCCTCTTTCCAACTAACATGC | 58 |
ITGA4 | 24 | AAAACCAGGTTGCTTTTTGC | ACCATGCTTGTTCCTTCCAC | 58 |
PDE1A | 7 | ACAGGGTGCACTGAAAGTCC | TTCTGGGCAGAGACAGATTG | 58 |
NCKAP1 | 11 | AGCAACATAAGAAAGGCAGGA | GAAGAATGCAGGGAGGTACG | 58 |
ZNF804A | 4 | CCACTGTTGCTGAAGATCCA | TTTGATCTGCTAATGGGACTGA | 58 |
FSIP2 | 12 | ATCCCTGATTGTGGCAATTC | AAGCAGACTGTTCAAGGGCTA | 58 |
COL3A1 | 30 | TAGTTCCCACCCAGCTGTTC | CAGCAGCACCCTGAAAATAA | 58 |
ANKAR | 16 | TGTCAATATAGCATACCCCTTTG | TTTTTGGCTTATTTCTCCAAGA | 58 |