Figure 1.
Culture and characterization of human dental follicle-derived stem cells (DFCs). (A) Morphology of DFCs in primary culture (P0) and at 3rd passage (P3). Plate-adhesion and fibroblast-like growth pattern were observed. Scale bar = 50 µm. (B) DFC at passage 3 showing expression of early transcription markers, Oct4, Sox2, and Nanog, by RT-PCR. (C) Growth curve for population doubling time (PDT) of DFCs during the 14 days of cell culture, which showed a favorable cell proliferation pattern of DFCs. Abbreviations: Oct4, octamer-binding transcription factor 4; Sox2, sex determining region Y-box 2.