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. 2016 Oct 18;13(11):841–852. doi: 10.7150/ijms.16573

Figure 3.

Figure 3

Lineage specific differentiation potential of DFCs. (A) Successful in vitro adipogenic and osteogenic differentiation of DFCs as identified by Oil red-O staining for lipid droplets and Alizarin red and von Kossa staining for mineralized nodules and calcium deposition, respectively. Scale bar = 100 µm. (B) Relative mRNA levels of differentiated cells compared to those of undifferentiated DFCs by RT-qPCR. Approximately 4-10 times and 10-20 times higher expression levels of lineage-specific mRNAs were detected in the differentiated adipogenic and osteogenic cells, respectively, compared to those in undifferentiated DFCs. Asterisk (*) represents statistically significant differences between the two groups (p < 0.05). Abbreviations: PPARγ, peroxisome proliferative activated receptor gamma; FABP4, fatty-acid-binding protein 4; LPL, lipoprotein lipase; RUNX2, runt related transcription factor 2; ON, osteonectin; BMP2, bone morphogenetic protein 2.