TABLE 3.

Percent abundance of cecal bacteria phyla and significantly altered taxa in male mice fed a 45%-fat diet with or without an ETWB supplement for 10 wk¹

	Control	ETWB	Percentage difference (ETWB relative to control)2	P3
Phylum
Tenericutes	0.08 ± 0.01	0.20 ± 0.04	150	0.0042
Bacteroidetes	30.8 ± 1.9	43.8 ± 2.7	42	0.0014
Firmicutes	63.6 ± 1.7	53.5 ± 3.0	−16	0.0079
Actinobacteria	1.19 ± 0.40	0.99 ± 0.28	−17	1.00
Verrucomicrobia	4.22 ± 0.54	1.49 ± 0.40	−65	0.0003
Proteobacteria	0.05 ± 0.02	0.01 ± 0.01	−80	0.0015
Taxon4
p__Firmicutes; g__Dorea	0.06 ± 0.01	0.30 ± 0.07	416	0.0008
p__Tenericutes; o__RF39	0.07 ± 0.01	0.19 ± 0.04	175	0.0056
p__Firmicutes; g__Adlercreutzia	0.09 ± 0.01	0.18 ± 0.02	99	0.0115
p__Bacteroidetes; f__Rikenellaceae	4.75 ± 0.43	9.41 ± 0.88	98	0.0010
p__Firmicutes; f__Ruminococcaceae	3.59 ± 0.62	5.81 ± 0.49	62	0.0057
p__Bacteroidetes; f__S24-7	26.1 ± 2.0	34.4 ± 2.3	32	0.0392
p__Verrucomicrobia; g__Akkermansia	4.22 ± 0.54	1.49 ± 0.40	−65	0.0007
p__Firmicutes; c__Clostridia	4.40 ± 0.47	1.50 ± 0.18	−66	<0.0001
p__Firmicutes; g__Streptococcus	0.12 ± 0.10	0.04 ± 0.36	−72	<0.0001
p__Firmicutes; g__Turicibacter	5.91 ± 0.88	1.08 ± 0.27	−82	0.0010
p__Firmicutes; o__Clostridiales	0.95 ± 0.18	0.08 ± 0.10	−91	<0.0001
p__Firmicutes; f__Peptostreptococcaceae	0.60 ± 0.17	0.01 ± 0.01	−98	0.0384

¹Values are means ± SEMs, n = 15/group. c_, class; ETWB, enzyme-treated wheat bran; f_, family; g_, genus; o_, order; p_, phylum.

²Percentage difference = [(ETWB − control)/control] × 100.

³Group comparisons were assessed by Mann-Whitney U tests. P values were adjusted for false discovery rate correction. Significance was set at an adjusted P value ≤0.05.

⁴Reported taxa included had a minimum of 0.05% mean abundance in each group and an adjusted P value ≤0.05. Bacteria are listed to the lowest level of classification (i.e., if the last taxon assignment is f_, “family” is the lowest level of classification).