TABLE 3.
Percentage abundances of cecal bacteria phyla and significantly altered taxa (ranked from highest to lowest by percentage difference) in male mice fed a 45%-fat diet with or without HAMRS2 supplementation for 10 wk1
| Control | HAMRS2 | Percentage difference (HAM RS2 relative to control)2 | P3 | |
| Phylum | ||||
| Tenericutes | 0.08 ± 0.01 | 0.31 ± 0.04 | 310 | 0.0002 |
| Actinobacteria | 1.19 ± 0.40 | 2.56 ± 0.80 | 115 | 0.06 |
| Bacteroidetes | 30.8 ± 1.86 | 54.2 ± 3.65 | 76 | 0.0001 |
| Verrucomicrobia | 4.22 ± 0.54 | 6.62 ± 0.87 | 57 | 0.0198 |
| Proteobacteria | 0.05 ± 0.02 | 0.03 ± 0.02 | −35 | 0.0198 |
| Firmicutes | 63.6 ± 1.66 | 36.2 ± 3.07 | −43 | <0.0001 |
| Taxon4 | ||||
| p__Bacteroidetes; f__Rikenellaceae | 4.75 ± 0.43 | 29.4 ± 4.15 | 520 | <0.0001 |
| p__Tenericutes; o__RF39 | 0.07 ± 0.01 | 0.33 ± 0.05 | 373 | 0.0003 |
| p__Firmicutes; f__Ruminococcaceae | 3.59 ± 0.62 | 11.00 ± 1.43 | 206 | 0.0003 |
| p__Firmicutes; f__Lactobacillaceae | 1.26 ± 0.53 | 2.94 ± 0.52 | 134 | 0.0020 |
| p__Verrucomicrobia; g__Akkermansia | 4.22 ± 0.54 | 6.45 ± 0.83 | 53 | 0.0348 |
| p__Firmicutes; g__Streptococcus | 0.12 ± 0.01 | 0.09 ± 0.02 | −23 | 0.0192 |
| p__Firmicutes; f__Lachnospiraceae; g__Ruminococcus | 0.53 ± 0.06 | 0.27 ± 0.04 | −48 | 0.0125 |
| p__Firmicutes; g__Oscillospira | 2.44 ± 0.19 | 1.00 ± 0.16 | −59 | 0.0010 |
| p__Firmicutes; f__Ruminococcaceae; g_Ruminococcus | 12.6 ± 0.85 | 4.61 ± 0.93 | −63 | 0.0001 |
| p__Firmicutes; f__Lachnospiraceae | 25.2 ± 1.27 | 7.73 ± 1.40 | −69 | <0.0001 |
| p__Firmicutes; c__Clostridia | 4.40 ± 0.47 | 0.93 ± 0.13 | −79 | 0.0001 |
| p__Firmicutes; o__Coriobacteriales | 3.35 ± 0.45 | 0.56 ± 0.19 | −83 | 0.0003 |
| p__Firmicutes; f__Peptostreptococcaceae | 0.60 ± 0.17 | 0.08 ± 0.05 | −86 | 0.0418 |
Values are means ± SEMs; n = 15 in the control group, n = 14 in the HAMRS2 group. c_, class; f_, family; g_, genus; HAMRS2, high-amylose-maize resistant starch type 2; o_, order; p_, phylum.
Percentage difference = [(HAMRS2 − control)/control] × 100.
Group comparisons were assessed by Mann-Whitney U tests. P values were adjusted for false discovery rate correction. Significance was set at an adjusted P value ≤0.05.
Reported taxa had a minimum of 0.05% mean abundance in each group and an adjusted P value ≤ 0.05. Bacteria are listed to the lowest level of classification (i.e., if the last taxon assignment is f_, “family” is the lowest level of classification).