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. 2016 Nov 22;7:446. doi: 10.3389/fphar.2016.00446

FIGURE 2.

FIGURE 2

Effect of α5-PAM on stress behavior induced by UCMS in female and male mice. (A) Timeline of experimental procedures. Tick marks indicate the beginning of a week. Depending on number of animals, behavioral testing took between 1 and 3 weeks. (B) Female mice treated with α5-PAM chronically had increased percentage of open arm entries relative to UCMS-VEH mice, with no effect of treatment in male mice. (C) No effect of either acute or chronic α5-PAM treatment in the OFT of either male or female mice after UCMS. (D) Treatment with acute and chronic α5-PAM reduced the latency to bite a food pellet in the NSF in female but not male mice. (E) Chronic α5-PAM treatment reduced the latency to bite a piece of cookie in the cookie test in female mice, while a trend in the same direction was observed following acute treatment. No significant effect of α5-PAM was detected in males. (F) Significant anti-stress effect of both acute and chronic α5-PAM treatment on female, but not male, Z-emotionality scores, Data represent mean ± SEM (N = 8–10 per group). ∗p < 0.05, ∗∗p < 0.01.