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. 2016 Oct 18;12(11):1289–1297. doi: 10.7150/ijbs.16405

Figure 5.

Figure 5

BAPN decreases tissue stiffness in Rip1-Tag2 mouse insulinomas. (A) The insulinoma sections of Rip1-Tag2 mice treated with normal water or BAPN water were subjected to immunofluorescence staining for collagen type І. Representative images and quantitation of immunofluorescence staining in the insulinoma sections of Rip1-Tag2 mice treated with BAPN or PBS. Rip1-Tag2 mice treated with BAPN exhibited much less type I collagen deposition than control mice. (B) Representative images and quantitation of Masson's staining of insulinoma sections from Rip1-Tag2 mice treated with BAPN or PBS. Collagen fiber deposition levels were determined by evaluating the blue areas as percentages of the total microscope area. (C) Representative images and quantitation of reticulin staining of insulinoma sections from Rip1-Tag2 mice treated with BAPN or PBS. Rip1-Tag2 mice treated with BAPN exhibited less reticular fiber deposition than control mice. (D) Hydroxyproline concentrations were detected in the insulinomas of 12-week-old Rip1-Tag2 mice treated with BAPN and PBS. Hydroxyproline levels were lower in the insulinomas of Rip1-Tag2 mice treated with BAPN than in the insulinomas of control mice. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Bar = 50 μm.