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LdChSA and LdChSB RNAi on chitin-containing structures in L. decemlineata. The dsegfp (the left column)-, dsChSA (the middle column)-, and dsChSB (the right column)-fed larvae are dissected and observed under a light microscope. The tracheae are visualized using light microscope before (A-F) and after treatment with 10 M of NaOH at 95 °C for 2 hrs (G-I). SP, spiracle; TR, tracheae; L, tracheal lumen; TA, taenidia.
