Figure 3.

Inhibition of Rg1 on H₂O₂-induced membrane blebbing and neurite retraction in PC12 cells. PC12 cells were pre-treated with different concentrations of Rg1 (0.1, 1 and 10 μM) for 12 h prior to 100 μM H₂O₂ treatment. NAC (500 µM) was used as positive control. (A) PC12 cell morphology. Bar, 10 μm. (B) PC12 cells membrane blebbing (C) The percentage of neurite length compared to control. (D) TEM of treated PC12 cells. Bar, 1 μm. Results were expressed as mean ± SD from three independent experiments (^##P< 0.01 versus control, ^*P< 0.05 versus H₂O₂-treated cells, ^**P< 0.01 versus H₂O₂-treated cells).