Figure 4.

Inhibition of Rg1 on H₂O₂-induced membrane blebbing and neurite retraction in neurons. Neurons were pre-treated with different concentrations of Rg1 (0.1, 1 and 10 μM) for 12 h prior to 100 μM H₂O₂ treatment. NAC (500 µM) was used as positive control. (A) Neurons morphology in each experimental group. Bar, 5 μm. (B) Immunofluorescence of MAP2. Nuclei staining was performed with DAPI. Bar, 20 μm. (C) Quantification of MAP2 fluorescence intensity in neurons. (D) The percentage of neurite length compared to control. Results were expressed as mean ± SD from three independent experiments (^##P< 0.01 versus control, ^*P< 0.05 versus H₂O₂-treated cells, ^**P< 0.01 versus H₂O₂-treated cells).