Figure 7.

Rg1 attenuates H₂O₂-induced caspase-3/ROCK1/MLC activation in PC12 cells. PC12 cells were pre-treated with different concentrations of Rg1 (0.1, 1 and 10 μM) for 12 h, and then co-cultured with H₂O₂ (100 μM) for another 12 h. Total cell lysates were subjected to western blotting. Protein level of caspase-3, cleaved caspase-3 (A), ROCK1, cleaved ROCK1 (B), ROCK2, cleaved ROCK2 (C), MLC and P-MLC (Ser-19) (D) in PC12 cells were measured using the relevant antibodies, and GAPDH as loading control. Results were obtained from three independent experiments and were expressed as mean ± SD (^##P < 0.01 versus control, ^*P < 0.05 versus H₂O₂-treated cells, ^**P < 0.01 versus H₂O₂-treated cells).