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. 2016 Oct 26;12(11):1394–1404. doi: 10.7150/ijbs.16517

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Detection of electrophoretic bands reacting with the antiserum against HarmCSP4 in crude extract of proboscis (P), pump (p) and eyes (E) of H. armigera. When a moth was allowed to drink from a solution of a fluorescent compound (A), strong fluorescence was observed in a cavity connected to the proboscis and situated just behind the eye, a pump for pushing a liquid through the proboscis (B) Cross-reactivity is observed in the proboscis sample, due to high concentrations of HarmCSP4 (Tables 1 and S2), and in the eyes, where HarmOBP11 (75% identical with HarmCSP4) has been detected in proteomic analysis (Tables 2 and S6). (C) Coomassie stained gel. (D) Western blot with both first antiserum against HarmCSP4 and HRP-linked secondary antiserum diluted 1:1000.