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. 2016 Nov 22;6:37751. doi: 10.1038/srep37751

Figure 3. Effect of silymarin on ERK/p38 MAPK pathway in CSE-exposed Beas-2B cells.

Figure 3

Cells were pretreated with silymarin (20 μM), U0126 (10 μM) or SB203580 (10 μM) before exposed to 6%CSE for 24 h. (A) Phosphorylated and total levels of ERK and p38 were measured by Western Blot. (B) Densitometry was performed and the ratio of p-p38/t-p38 and p-ERK/t-ERK were calculated. Results are representative of three independent experiments. Values are expressed as mean ± SEM (n = 3). *P < 0.05 with respect to the control group; #P < 0.05 with respect to the CSE-exposed group.