Figure 4. GC-MS analysis of lipid droplet free fatty acid content and expression heat maps of genes associated with unsaturated fatty acid biosynthesis, arachidonic acid metabolism, and fatty acid metabolism in P. brassicae.
(A) GC-MS analysis of the free fatty acid methyl ester composition of lipid droplets. Note: 1. C16:0; 2. C18:0; 3. C18:1Δ9; 4. C18:1Δ11; 5. C18:2Δ9, 12; 6. C20:4Δ5, 8, 11, 14; 7. C20:5Δ5, 8, 11, 14, 17; 8. 2, 4-bis (1,1-dimethylethyl)-phenol; 9. Benzenepropanoic acid-methyl ester; and 10. C32H66. (B) Gene expression of genes in KEGG categories involved in unsaturated fatty acid biosynthesis, arachidonic acid metabolism and fatty acid metabolism. A colored bar indicating the normalized reads per LOG2 (RPKM) accompanies the expression profile.