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. 2016 Nov 22;6:37336. doi: 10.1038/srep37336

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Copyright © 2016, The Author(s)

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The fresh-made mitochondria were pre-incubated with Calcium indicator Quest Fluo-8^TM, AM or membrane potential dye Rhodamine 123 for 15 min and washed three times using potassium chloride (KCl) media containing 5 mM succinate. After recording the baseline, the mitochondria were perfused with 10 uM Ca²⁺ or 5 uM FCCP. (A) Serotonin hydrochloride (5-HT) decreases mitochondrial Ca²⁺ uptake. (B) Serotonin creatinine sulfate (5-HT(H₂SO₄)) attenuate Ca²⁺ uptake. (C) The percentage of Ca²⁺ uptaken by mitochondria of 5-HT groups (n = 6) and 5-HT (H₂SO₄) groups (n = 6). (D) Ca²⁺ induced mitochondrial Ca²⁺ uptake can be inhibited by RUR (n = 5). (E) Transmitochondrial potential (measured with 5 nM Rh-123) in control and 100 uM 5-HT pre-incubated mitochondria. 5 uM FCCP (Protonophore trifluoromethoxy carbonyl cyanide phenylhydrazond) was added to depolarize the mitochondria (n = 3).