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. 2016 Nov 22;6:37395. doi: 10.1038/srep37395

Figure 1. Mutation types and frequencies in T0 plants with 10 target sites.

Figure 1

(A) Mutation and pollen sterility frequencies in T0 plants with 10 target sites. The target site of TMS5b displayed the highest mutation frequency. The TMS5ab construct gave rise to a higher percentage of plants with pollen sterility compared to the other constructs. The number preceding “,” is the total number or frequency of mutations, whereas the number following “,” is the number or ratio of homozygote plants. (B) CRISPR/Cas9-induced mutation types and frequencies. In all types of induced mutations, single-nucleotide insertions were most frequently detected. The largest deletion was 253 base pairs long. (C) TMS5ab construct-induced mutation frequencies in 11 cultivars of two different rice subspecies. Excluding YNSM, the mutation frequencies of TMS5a in the other ten cultivars were all higher than 72.72%.

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