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. 2016 Nov 22;9:126. doi: 10.3389/fnmol.2016.00126

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Copyright © 2016 Kasai, Kakihara, Miura, Okada, Hayata-Takano, Hazama, Niu, Shintani, Nakazawa and Hashimoto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-34a expression in parvalbumin-positive neurons in the TRN and cortical layer V. Double FISH was performed using miR-34a and parvalbumin (PV) probes. DIG-labeled LNA-modified probes for miR-34a and fluorescein-labeled RNA probes for PV were hybridized at 48°C. Detection was carried out using the TSA Plus fluorescent kit for PV (green), followed by the TSA Plus biotin kit and Texas red streptavidin for miR-34a (red). The insert images are a higher magnification of the boxed brain areas including the TRN. EP, entopeduncular nucleus; GP, globus pallidus. Scale bars, 1 mm.